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Question: 1. Add ampicillin to the flask containing 30 mL of LB to a final concentration of 50 μg/mL. 2. A…

Question: 1. Add ampicillin to the flask containing 30 mL of LB to a final concentration of 50 μg/mL. 2. A...

Question: 1. Add ampicillin to the flask containing 30 mL of LB to a final concentration of 50 μg/mL. 2. A...

1. Add ampicillin to the flask containing 30 mL of LB to a final concentration of 50 μg/mL. 2. Add 0.8 mL of LB to your semi-micro cuvet and use this as the blank to zero the spectrophotometer. Then add 0.4 mL of the E. coli culture, cover the cuvet with a small piece of parafilm, mix by inversion, and record the OD600 of the 3-fold dilution of cells. Given that 1 OD unit at 600 nm is equivalent to about 1 x 10 cells/mL, calculate the appropriate volume of the culture to add to 30 mL of LB medium in the 125 mL flask to obtain a cell density of about 1 x 108 cells/mL. Have your calculation checked by a teaching assistant before proceeding. ● Add the volume ofcells calculated above to the 30 mL of LB containing 50 μg/mL ampicillin Measure and record the OD600 of the culture (0.8 mL culture in semi-micro cuvet.)

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