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Question: 3. The central dogma of molecular biology describes the flow of genetic information in an organis…

3. The central dogma of molecular biology
describes the flow of genetic information in an organism. Number
the descriptions below in the proper order, from the
earliest event (1) to the latest event (8) in the
expression of the GFP gene in bacteria. Assume that transcription
is completed prior to translation. Hint: Reference Fig. 4.17
from “How Life Works” textbook to review translation section.

[8 pts]

_____        GFP folds into
complete tertiary structure and exhibits fluorescence.

_____       
tRNAMet recognizes the AUG start codon.

_____        Methionine
detaches from tRNAMet during peptide bond formation.

_____        RNA Polymerase
recognizes a terminator, releasing full-length GFP mRNA.

_____        Full-length GFP
is produced and ribosome disassociates from mRNA template.

_____        RNA Polymerase
binds to the GFP DNA promoter and initiates transcription.

_____        The ribosome
encounters a UAA codon and a release factor binds the “A” site.

_____        An uncharged
tRNA (formerly tRNAMet) is ejected from the “E”
site.

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