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A hexapeptide of the sequence A-H-R-E-D-G is subjected to a trypsin cleavage. The resulting fragments must then be purified. Please state the products of the cleavage. If the cleaved portion containing the histidine residue was subjected to a pH of 1 and portion containing the aspartate was subjected to a pH of 10, which resulting peptide would be purified with a DEAE column and which with a CM? How would you then elute them?
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