Different kinds of DNA polymerase work at different paces. Let’s
say the kind you’re using in your PCR can extend 500 base pairs per
minute (this is relatively slow for DNA polymerases). If the region
between your primers is 750 base pairs, how long should you set
your extension time? What happens if you calculate incorrectly and
you set your extension time to 1 minute? What do you think happens
if you set your extension time to 2 minutes?
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