24/7 Study Help

Studykind Will Help You Write Your Essays and Term Papers

From initial topic to finished paper

Want answers to the assignment Below? 

For premier support Text or Whatsapp Ivanna at +1(478) 377-7377 

Question: Effect of heat.-Heating for 1 hour at 56 degree or 80 degree C. has no effect on the antibacteria…

Question: Effect of heat.-Heating for 1 hour at 56 degree or 80 degree C. has no effect on the antibacteria...

Effect of heat.-Heating for 1 hour at 56 degree or 80 degree C. has no effect on the antibacterial power of penicillin. Boiling for a few minutes hardly affects it (see Table II). Boiling for 1 hour reduces it to less than one quarter its previous strength if the fluid is alkaline, but if it is neutral or very slightly acid then the reduction is much less. Autoclaving for 20 minutes at 115 degree C. practically destroys it. Effect of filtration.-Passage through a Seitz filter does not diminish the antibacterial power. This is the best method of obtaining sterile active mould broth. Solubility.-It is freely soluble in water and weak saline solutions. My colleague, Mr. Ridley, has found that if penicillin is evaporated at a low temperature to a sticky mass the active principle can be completely extracted by absolute alcohol. It is insoluble in ether or chloroform. Rate of development of inhibitory substance in culture.-A 500 e.e. Erlenmeyer flask containing 200 e.e. of broth was planted with mould spores and incubated at room temperature (10 degree to 20 degree C.). The inhibitory power of the broth to staphylococcus was tested at intervals. After 5 days complete inhibition in 1 in 20 dilution. Grown at 20 degree C. the development of the active principle is more rapid and a good sample will completely inhibit staphylococci in a 1 in 500 or 1 in 800 dilution in 6 or 7 days. As the culture ages the antibacterial power falls and may in 14 days at 20 degree C. have almost disappeared. The antibacterial power of penicillin falls when it is kept at room temperature. The rate of this fall can be seen from Table I. If the reaction of penicillin is altered from its original pH of 9 to a pH of 6 middot 8 it is much more stable. The small drops of bright yellow fluid which collect on the surface of the mould may have a high antibacterial titre. One specimen of such fluid completely inhibited the growth of staphylococci in a dilution of 1 in 20,000 while the broth in which the mould was growing, tested at the same time, inhibited staphylococcal growth in 1 in 800. If the mould is grown on solid medium and the felted mass picked off and Jack needs to prevent bacterial growth in a solution of nutrient media, and it needs to prevent Staphylococcus contaminants from growing for at least 15 days. a. According to figure 1 of paper, how much Penicillin extract should he add if he needs to make 1600 mL of nutrient media? i. What volume of Penicillin should he add? mL ii. What volume of nutrient media should he add? mL

Essay Writing Service Ready to Help Online 24/7

Hire a professional just from $9.99

Click here

HOME TO CERTIFIED WRITERS

Why Place An Order With Us?

  • Certified Editors
  • 24/7 Customer Support
  • Profesional Research
  • Easy to Use System Interface
  • Student Friendly Pricing

Have a similar question?

Need a previously written solution for this question?

Services that we offer

Your Paper Is In Safe Hands

PLAGIRAISM FREE PAPERS

All papers we provide are well-researched, properly formatted and cited.

TOP QUALITY

All papers we provide are well-researched, properly formatted and cited.

HIGHLY SECURED

All papers we provide are well-researched, properly formatted and cited.

Related Assignments

Study more efficiently

Quickly get your essay from idea to final graft save time, money and effort.

Boost your grade

Get through your toughest problem and learn how to solve them from expert writers

Get on board with the best writers.

“All our writers are certified professionals, holding Masters Degrees and above.”

Our company has worked successfully in the field of academic assistance for over 11 years. We consistently upgrade our goals to improve the quality of service we provide and to maximize customer satisfaction. Success has driven us forward to an innovative concept. Our working experience, customer feedback, and market resources have brought about the creation of an exclusive online service:

Chat now
Powered by Studykind
Hello! Welcome to to our whatapp support.

We offer READY solutions, HIGH QUALITY PLAGIARISM FREE essays and term-papers.

We are online and ready to help.