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Question: Measure the diameter of the field of view. Once you determine the size of the field of view for a…

Question: Measure the diameter of the field of view. Once you determine the size of the field of view for a...

Measure the diameter of the field of view. Once you determine the size of the field of view for any combination of ocular and objective lenses you can determine the size of any structure within that field. a. Rotate the 4% objective into position and remove the letter slide. b. Place a clear ruler on the stage, and focus on its edge. c. The distance between two lines on the ruler is 1 mm. What is the diameter (mm) of the field of view? D. Convert this measurement to micrometers (gm), a more commonly used unit of measurement in microscopy (1 mm-1000 mu m). e. Measure the diameters of the field of view for the 10 times and 40 times objectives, and enter all three in the spaces below to be used for future reference, 4 times = 10 times = 40 times = f. What is the relationship between the size of the field of view and magnification? Determine spatial relationships. The depth of field is the thickness of the specimen that may be seen in focus at one time. Because the depth of focus is very short in the compound microscope, focus up and down to clearly view all planes of a specimen. a. Rotate the 4 times objective into position and remove the ruler. Take a slide of crossed threads, wipe it with a Kimwipe*, and place the slide on the stage. Center the slide so that the region where the two threads cross is in the center of the stage opening. b. Focus on the region where the threads cross. Are both threads in focus at the same time? c. Rotate the 10 times objective into position and focus on the cross. Are both threads in focus at the same time? Does the 4 times or the 10 times objective have a shorter depth of field?

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