Prepare 100 ml of a 0.5 M NaOH solution. MW of NaOH is 40.0 Prepare 500 ml of a 2.0M NaCl/0.5M NaOH solution. MW of NaCl is 58.44 Prepare 100 ml of a 10% SDS solution. MW of SDS is 288.4 Using the following stock solutions, 1.0M tris-HCl, 1.0M NaCl, 0.5M EDTA, prepare 500 ml of a 0.2M Tris-HCl/0.4M NaCl/0.1M EDTA solution. From a stock solution of 3.3 mg/ml stock of DNA, make a 250 ul solution of 1.5 ng/ul DNA. A solution contains 2.5 times 10^8 bacteria/ml. An aliquot of 10 ul is diluted into 10 ml to give solution A. 500 ul of solution A is dilluted into 10 ml of give solution B. 1 ml of solution B us spread on a plate. How many colonies are expected given that a colony arises from one bacterial cell.