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Question: Using the steps below, you can calculate the theoretical maximal yield of your plasmid DNA (in mi…

Using the steps below, you can calculate the theoretical maximal
yield of your plasmid DNA (in micrograms) that you could obtain
from your 1.5ml overnight cell culture if the culture had OD600=3.0
Assume that the unknown plasmid is 3 kb long.

a. First assume that each E.coli cell carries 50 copies of the
unknown plasmid. How many plasmid DNA molecules are
theoretically in the 1.5 ml overnight culture? (first figure out
how many cells are in the 1.5ml culture using the conversion of
OD600 to cells/ml) Show your calculations.

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