Various features of the GFP gene are labeled with letters A-H.
Match the letters below with the appropriate number feature from
the list below. Each letter has only one number match. Numbers are
used only once, and not all numbers will be used.
1b. Mutations in DNA can change the
corresponding mRNA and the protein product produced. In which of
the features from question 1a (#1-15) would you expect to find a
mutation that reduced the recruitment of RNA Polymerase? Explain
your answer.
1c. In which of the numbered features (#1-15)
would you expect a mutation that prevented the methionine tRNA
(3’-UAC-5’) from binding its target sequence during translation?
Explain your answer.
1. In Experimental Biology we will investigate the expression of Green Fluorescent Protein (GFP) in bacterial cells. The image below represents a simplified schematic of the gene expressed in bacteria (using 2 horizontal lines for DNA), along with the corresponding mRNA product (one horizontal line). The black box represents the region that encodes for a protein. DNA GFP GENE RNA gene are labeled with letters A-H. Match the letters below 1a. Various features of the GFP with the appropriate number feature from the list below. Each letter has only one number match. Numbers are used only once, and not all numbers will be used. [8 pts] Letter Label Feature 1. Amino acid 2. Stop codon 3. Start codon 4. ORF (open reading frame) 5. tRNA 6.3′-unstranslated region 7. Transcription start site 8. PolyA tail 9. Promoter 10. tRNA 11. Splicing branch point 12. 5′-untranslated region 13. Transcription termination site 14. Intron 15. A (aminoacyl)-site
