What are the three broad methods used by microbiologists to identify microbes to genus and species level? What constitutes microbial transport medium. What are the three requirements of a good microscope? What does resolution show? What does contrast provide? And what determines contrast? What is the effect of differences in refractive index? What image do we see through the ocular lens of a microscope? And what is important about it? How do we calculate magnification of a microscope? If we have an ocular of magnification power 10X and an objective lens 40X in place what magnification of our sample will we see? What is the definition of resolution? How do we determine resolving power? Why do we place a drop of oil between the cover-slip on top of the specimen and the oil immersion lens of a compound light microscope? How can we increase the resolving power of a microscope. We can we compensate for the lack of contrast between a cell and its background? List the names of the four types of visible-light microscopes. What are basic stains, what do they do? Name four basic stains. What is another name for a negative stain? What do these stains do? And name two. What is the concept of the differential stain? Give examples. How does colony morphology of the Streptococci differ from that of the Staphylococci? What is a very simple description of an antibody? What is a good sample to use the direct fluorescence assay on? What are the exceptions. What kind of assay is the Streptococcus dipstick test that we discussed? What are the Five 1’s of microbial cultivation in order of operation? What are the steps involved in inoculation? What is involved in incubation? How is isolation achieved? What does inspection involve?
