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Question: You have an original concentration of 1.2 times 10^7 org/mL and you want to obtain a final concen…

Question: You have an original concentration of 1.2 times 10^7 org/mL and you want to obtain a final concen...

You have an original concentration of 1.2 times 10^7 org/mL and you want to obtain a final concentration of 3 times 10^2 org/mL. Draw a dilution scheme with the following information on your diagram: a. the sample volume transferred and the volume of the diluent at each step RESTRICTION: Final volume for each tube can be no more than 5 mL and no less than 2 mL. b. the dilution at each step c. the dilution factor at each step d. the total dilution factor at each step e. the concentration at each step Then calculate how many CFUs you would expect to see if you plated a total volume of 200 mu L of the last dilution tube.

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