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Question: You plan to use a PCR based assay to determine whether 3 mosquitoes that you have collected are i…

Question: You plan to use a PCR based assay to determine whether 3 mosquitoes that you have collected are i...please be more specifictly

You plan to use a PCR based assay to determine whether 3 mosquitoes that you have collected are infected with West Nile virus. You have available to you three different virus-specific primers. The primers recognize sequences at the following nucleotide positions on the viral genome: primer 1: 155-176 primer 2: 745-720 primer 3: 926-905 The 5′ and 3′ ends of the genome are indicated on the long line The first nucleotide at the 5′ end of the genome is position #1 The 5′ ends of the primers are indicated (a) List all the steps you would use in the PCR based assay you develop for the detection of viral-specific sequences. Briefly describe what is occurring at each step. You may use any of the primers listed above. Indicate which primers you choose to use in your diagnostic assay.

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