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Question: 3. Control of Trehalase gene expression You are interested in examining the regulation of the gen…

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Question: 3. Control of Trehalase gene expression You are interested in examining the regulation of the gen...

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3. Control of Trehalase gene expression You are interested in examining the regulation of the gene that encodes an enzyme, Trehalase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Trehalase gene and are involved in regulating the transcription of the Trehalatgene. You make mutations in the TreA and Treß genes to create cells lacking these genes (and thus lacking TreA and TreB proteins), observe what happens to transcription of the Trehalase gene, and obtain the results in the table below. Transcription of Trehalase in different medium Glucose Trehalose Glucose + Trehalose normal cells lacking TreA cells lacking TreB OFF ON OFF cells lacking both TreA & TreBOFFOFFOFF onhy OFF OFF OFF only ON ON OFF is TreA an activator or repressor? Explain its role in controlling Trehalase expression with and without glucose present. is TreB an activator or repressor? Explain its role in controlling Trehalase expression with and without trehalose present. a) b)

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