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Question: Hey This is Genetics. I really need help with numbers 1 to 4. Detailed explanation would help. Th…

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Question: Hey This is Genetics. I really need help with numbers 1 to 4.Detailed explanation would help. Th...

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Hey This is Genetics. I really need help with numbers 1 to 4.
Detailed explanation would help. Thanksss

0a 03 aco1 BI 513 Homework 2 – Due by 6 pm on Monday 11/13/17 – page 2 Below is a table of some of the mutations found by sequencing the ade2 alleles identified in class this year. The base number is according the numbering on the ADE2 sequence PDF file (The start codon is at base 101 of the PDF file.) Questions 1- 4 are based on this data. Mutant location of mutationMutation on sequence (base 120 410 654 671 881 1374 1413 to A C to T G to A G to A to to C to T G to A C to T to G to A G to A G to A 1505 1515 1517 1518 1553 1554 G to 1623 G to A 1) For each mutant, determine the effect of the mutation on translation (same amino acid, different amino acid, other). You should present your answers in a table. 2) Based on information you can find in the literature regarding about EMS, discuss each mutation as to whether it is consistent with EMS being the mutagen. (Remember to consider both strands of the helix.) Are there any target biases reported for EMS in terms of the surrounding sequence context? Are our results consistent with published findings? 3) Which mutant (or mutants) is the best candidate to be a true “null” allele? 4) Based on results from this year and other years, greater than 34 of ADE2 missense mutations are found between bases 1300-1800, the part of the gene that codes the C- terminal region of the ADE2 protein. Discuss this observation in relation to conserved regions of the phosphoribosylaminoimidazole carboxylase homologs.

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